Table 1. Reagents and Conditions Used for Immunohistochemistry.
Antibody | Clone/Source | Dilution | Platform and Pretreatment |
---|---|---|---|
CD20 | L26a | 1:1000 | BOND-MAX, ER2 retrieval |
CD3 | Rabbit Polyclonalb | 1:100 | BenchMark XT, Standard retrieval |
CD10 | 56C6c | 1:20 | BOND-MAX, ER2 Retrieval |
BCL2 | 124a | 1:50 | BenchMark XT, Standard retrieval |
BCL6 | GL191E/A8b | 1:100 | BenchMark XT; Standard retrieval |
HGAL | MRQ-49b | 1:100 | BOND-MAX, ER2 retrieval |
IgM | Polyclonala | 1:1250 | BenchMark XT; Standard retrieval |
IRF4 | MUM1Pa | 1:40 | BOND-MAX, ER1 retrieval |
Ki67 | MIB-1a | 1:200 | BOND-MAX, ER2 retrieval |
CD21 | IF8a | 1:80 | BOND-MAX, ER2 retrieval |
CD23 | 1B12c | 1:50 | BOND-MAX, ER2 retrieval |
D2-40 | D2-40a | 1:80 | BOND-MAX, no retrieval |
Kappa | Rabbit polyclonala | 1:1000 | BenchMark XT; Protease 2 retrieval |
Lambda | Rabbit polyclonala | 1:4000 | BenchMark XT; Protease 2 retrieval |
aDako, Carpinteria, CA
bCell Marque, Rocklin, CA
cNovocastra, Newcastle upon Tyne, UK