Table 1. Comparison of identified (photo)-cross-links in full-length PPAR-β/δ and PPAR-β/δ LBD.
For the LBD, cross-links are summarized for all reagents used in this study, while for full-length PPAR-β/δ only BS2G cross-links are presented; { denotes the N-terminus of the protein; } denotes the C-terminus of the protein.
| PPAR-β/δ ligand-binding domain (LBD) | PPAR-β/δ full-length | ||||||
|---|---|---|---|---|---|---|---|
| Cross-linked amino acids | Free | GW0742 | GW1516 | Cross-linked amino acids | Free | GW0742 | GW1516 |
| {167+S181/K182 | X | X | X | {+K107 | X | X | |
| {167+K182 | X | X | X | {+S116/K118 | X | ||
| {167+K190 | X | X | X | {+K121/K122 | X | X | X |
| {167+T195/K196/K197 | X | {+K122 | X | X | X | ||
| {167+K196/K197/K198 | X | X | X | {+K126 | X | X | |
| {167+K197/K198 | X | X | X | {+K155 | X | X | |
| {167+K198 | X | {+K197/K198 | X | X | X | ||
| {167+S201 | X | {+K198 | X | X | |||
| {167+K231 | X | {+K423 | X | ||||
| {167+E264 | X | X | X | K82+K121/K122 | X | ||
| {167+K267 | X | X | K107+K155 | X | X | ||
| {167+K324 | X | X | K118+K122 | X | X | X | |
| {167+E331 | X | K118+K126 | X | X | |||
| {167+E373 | X | X | X | K118+K134 | X | ||
| {167+D377 | X | X | X | K118+S181/K182 | X | ||
| {167+K404 | X | X | K118+K198 | X | |||
| {167+K423/K424 | X | X | K121+K126 | X | X | X | |
| {167+K440 | X | K122+K126 | X | X | X | ||
| {167+}443 | X | K122+K134 | X | X | X | ||
| {167/G167/S168/Q169/Y170+X180 | X | K122+K155 | X | ||||
| X180+I379 | X | X | X | K122+K157 | X | ||
| S181/K182+K197/K198 | X | X | X | K126+K134 | X | ||
| S181/K182+K198 | X | X | K126+K155 | X | |||
| K182+K197 /K198 | X | X | X | K134+K197/K198 | X | X | |
| K190+K197/K198 | X | X | X | K155+K182 | X | ||
| K190+K198 | X | X | X | K155+K190 | X | ||
| T195/K196/K197+K198 | X | X | K155+K197 | X | X | X | |
| K197/K198+S201 | X | X | X | K155+K197/K198 | X | ||
| K197/K198+K206 | X | X | X | K155+K404 | X | ||
| K197/K198+S208 | X | X | X | K157+K197/K198 | X | ||
| K197/K198+K206/S208/T210 | X | K190+K197/K198 | X | ||||
| K197/K198+K324 | X | K197/K198+S201 | X | X | X | ||
| K197/K198+K423/K424 | X | K198+S201 | X | X | X | ||
| K197+K424 | X | K324+K423/K424 | X | ||||
| K198+S201 | X | X | X | K423+K440 | X | X | |
| K198+K206 | X | X | |||||
| S201/T204/K206/S208+K302 | X | ||||||
| K226+Y249/K241 | X | ||||||
| K241+K423/K424 | X | ||||||
| S298/K302+K400 | X | ||||||
| K324+K423/K424 | X | X | X | ||||
| K324+K424 | X | X | |||||
| I330+X443 | X | ||||||
| K333+K423/K424 | X | ||||||
| M419+X443 | X | X | X | ||||
| K423+D441 | X | X | X | ||||
| K423/K424+T425/T427/T429/S430 | X | ||||||
| K423/K424+K440 | X | X | X | ||||