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Heterozygous (GC alleles with both 297and 244 bp amplicons) and homozygous (G with only 297 bp amplicon). The size of the common outer amplicon was 486 bp. Varieties used: J-Jakar, Y-Chumey, M- C8816 Malong, PI-PI481672, D-Donan, G-Golden, H-Hei Qiao-4, I-Ishisoba, O-01Z100014, K-C9717 Kuer, P3-PI481673, X-Xinong 9909, Q—Q000120, H-Hei Feng, P5-PI427235.
