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. 2016 Mar 18;11(3):e0151761. doi: 10.1371/journal.pone.0151761

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© 2016 Barsotti et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — The expression of intracellular IL-10 and surface markers CD24, CD38 and CD27 were determined by flow cytometry after in vitro stimulation of PBMC for 5h with CpG + PIB. Chronic activated T cells were determined in PBMC by the expression of surface markers CD4, CD8, CD38 and HLA-DR without stimulation and analyzed by flow cytometry. sCD14 levels in plasma were determined by ELISA. Correlation of CD19⁺CD24^hiCD38^hi IL-10⁺ cells with chronic activated (A) CD4⁺ and (B) CD8⁺ T cells in 43 CVID patients. Correlation of CD19⁺CD24^hiCD27⁺ IL-10⁺ cells with chronic activated (D) CD4⁺ and (E) CD8⁺ T cells in 43 CVID patients. Lines represent linear regression analysis. Correlation of CD19⁺CD24^hiCD38^hi IL-10⁺ (C) and CD19⁺CD24^hiCD27⁺IL-10⁺ (F) B cells with plasma levels of sCD14 in 41 CVID patients. Lines represent linear regression analysis. R and P values were calculated by Spearman’s rank order correlation test.