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. 2016 Mar 1;5(4):476–487. doi: 10.5966/sctm.2015-0108

Figure 1.

Figure 1.

Generation of human immature dorsal root ganglia neuron (iDRG) cells for toxicity testing. Pluripotent stem cells were differentiated in a two-step procedure, as indicated. (A–D): Immunocytochemical characterization of hESC-derived cells. Labels are color keyed to images. (E): Proliferating cells (EdU+) were quantified (mean ± SEM; n = 3). (F–I): Immunocytochemical characterization of iDRG cells after thawing. Labels are color keyed to images. Scale bars = 100 µm (A) and 50 µm (B–D, F–I). Abbreviations: BDNF, brain-derived neurotrophic factor; DoD, day of differentiation; GDNF, glia-derived neurotrophic factor; hESC, human embryonic stem cell; NGF, nerve growth factor.