Quantification of calcium signaling of immature dorsal root ganglia neuron cells. (A): Intracellular calcium levels measured on DoD7 after depolarization. Each cell is depicted by a circle. ***, p < .001. (B): Before depolarization by KCl, cells were pretreated with Verap (100 µM) or Nifed (100 µM; n = 3; ***, p < .001). Abbreviations: DMSO, dimethyl sulfoxide; HBSS, Hanks' buffered salt solution; Nifed, nifedipine; rel., relative; Verap, verapamil; Verat, veratridine.