Figure 5.

Profiling and quantification of negative and positive control toxicant effects on immature dorsal root ganglia neuron neurites. Tool compounds were used as in (4C). (A): Negative control. (B): Unspecific toxicants. (C): Guanylyl cyclase inhibitor as pathway-specific control. *, p < .05; **, p < .01; ***, p < .001 versus untr. cells. (D): Test of reversibility. After 24 hours of exposure, ODQ was washed out, and neurites were measured again 24 hours later (n = 3–4). (E): ROCK inhibitor as pathway-specific control for accelerated growth. (F): Positive controls (drugs causing peripheral neuropathy). Scale bars = 50 µm. Abbreviations: SDS, sodium dodecyl sulfate; untr., untreated.