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. 2016 Mar 3;5(4):427–439. doi: 10.5966/sctm.2015-0109

Figure 2.

Figure 2.

Screening for cell surface proteins in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) with altered expression during senescence. (A): Flow cytometric analysis of both early-stage (passage [P] 4) and late-stage (P10) hUCB-MSCs based on the cell surface expression of putative MSCs markers. (B): Heat map analysis showing downregulated cell surface proteins at a late passage (P10) compared with those observed at an early passage (P4). (C): To confirm the downregulation of cell surface proteins shown in panel B, the protein expression levels of CD47, CD71, CD106, CD146, CD165, CD274, and EGFR were measured by flow cytometry at early (P4) and late (P10) stages in hUCB-MSCs from 25 different donors (mean ± SD; n = 25; ∗, p < .05; ∗∗, p < .01). CD146 expression showed the most significant decline in late-stage cells (black box). (D): CD146 expression was quantified by using flow cytometry at the indicated passages (mean ± SD; n = 3; ∗∗, p < .01). Abbreviation: EGFR, epidermal growth factor receptor.