Figure 3.
Immunophenotyping results of sorted in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs), based on CD146 expression. (A): The percentage of CD146+ hUCB-MSCs was analyzed, and CD146+ and CD146− populations were sorted by flow cytometry. The plots shown indicate the CD146 staining profile (filled graph) versus isotype-control staining profile (unfilled graph; mean ± SD; n = 3). (B): The immunophenotypic characteristics of CD146+ and CD146− cells were examined by flow cytometry. Both CD146+ and CD146− cells were strongly positive for the MSC-specific surface markers CD29, CD73, CD90, CD105, and CD166, and they were negative for CD14 and CD45 (mean ± SD; n = 3).