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. 2016 Mar 18;11(3):e0151894. doi: 10.1371/journal.pone.0151894

Fig 1. Addition of non-essential amino acids extended the chronological lifespan of a prototrophic yeast strain.

Fig 1

(A), (B) Chronological lifespan and cell growth in a synthetic medium with or without amino acids. YOM36 cells grown in SD medium were transferred to SD or SC media and CFUs and cell density of the cultures were measured at the indicated times. (C) Effect of late addition of amino acids. Amino acid mixture or water was added to cultures of YOM36 cells grown in SD for the indicated times and chronological lifespan assay was performed as in (A). (D) Effect of media swap on chronological lifespan. The original media of the cells grown as in (A) and (B) were swapped at day 3. Error bars represent the means ± SD (n = 3). (A) P<0.01; (B) P<0.01; (C) P<0.01 (Day 1 water vs Day 1 amino acids; Day3 water vs Day 3 amino acids), P<0.05 (Day 5 water vs Day 5 amino acids); (D) P<0.01 (SD vs SD→SC; SC vs SC→SD).