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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Semin Cell Dev Biol. 2016 Jan 15;51:54–63. doi: 10.1016/j.semcdb.2016.01.016

Figure 2.

Figure 2

Buccopharyngeal membrane rupture. A) Sagital section through the middle of the head showing the buccopharyngeal membrane. Cells are labeled with phalloidin (green) and the red is autofluorescence. Phalloin labels F-actin which is located along membranes of cells. scale bar=33 μm. B) Frontal view of a face as the buccopharyngeal membrane perforates or ruptures. scale bar=130 μm. C–E) Frontal views of phalloidin labeled buccopharyngeal membranes before (C) and during (D,E) perforation. scale bar = 80 μm. F) Schematic of embryonic faces at stage 40, showing the stages of buccopharyngeal membrane rupture and presenting the hypothesis that biomechanical forces are important for this process. Abbreviations: BM; buccopharyngeal membrane.