Skip to main content
. Author manuscript; available in PMC: 2017 Mar 17.
Published in final edited form as: Mol Cell. 2016 Feb 18;61(6):821–833. doi: 10.1016/j.molcel.2016.01.020

Figure 4. Mbnl motifs are enriched and conserved within localized distal ALEs, and Mbnl promotes RNA localization to projections.

Figure 4

A) Enrichment of 6mers (hexanucleotides) between neurite distal and soma proximal UTRs and conservation of 6mers between mouse and human. Conservation is measured by a z-score representing the number of SD above the mean conservation of 50 control 6mers matched for CpG and C+G% content, in neurite distal UTRs. B) Metagene analysis of Mbnl motif frequency across UTRs from indicated classes (excluding the last 50 nt to exclude PAS motifs). These classes correspond to those defined in Figure 2. C) Relative CLIP-seq cluster densities in the UTRs of distal and proximal ALEs. Control UTRs consist of randomly sampled UTRs from all ALE events that were not differentially localized. Error bars are the standard error of random samplings of controls. D) Change in LR upon Mbnl knockdown for genes that were (blue) or were not (pink) localized in the control sample. E) Mbnl motif frequency across 3′ UTRs of ALEs as a function of the change in localization of that ALE in cortical neurons from Mbnl1 / Mbnl2 DKO mice. ALEs were classified by their ΔΔψ values as described in Supplemental Methods. Error bars represent +/− SEM. See also Figure S4, Table S3.