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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: ACS Chem Biol. 2015 Nov 19;11(1):193–199. doi: 10.1021/acschembio.5b00749

Figure 4.

Figure 4

Effect of esterified compounds on iron metabolism and collagen secretion. MDA-MB-231 breast cancer cells were treated with deferoxamine (DFO), ethyl dihydroxybenzoate (EDHB), biheteroaryl compounds, or vehicle (DMSO) and then analyzed with an immunoblot (*, p < 0.05). (A) Effect of esterified biheteroaryl compounds on iron metabolism. Blots are representative of at least 3 replicates. (B) Densitometric quantitations (n ≥ 3) corresponding to the blots in panel A and normalized to β-actin. The dose of EDHB (500 µM) is known to diminish collagen secretion significantly.6 (C) Effect of esterified biheteroaryl compounds on collagen secretion into conditioned media. Blots are representative of at least 5 replicates and quantitations (right) are normalized to total protein using the Ponceau S stained blot.