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Images of EGFP (green) and Hoechst 33,342 (blue) fluorescence were acquired at 40x with an automated widefield microscope (see Materials and methods). Each panel is independently contrast stretched. The identities of the tagged gene for each clone are listed in Supplementary file 1. Clone order is random with respect to location pattern. The untagged NIH 3T3 (upper left) was assigned as clone 46.
