Figure 5. Degree of perturbation for all experimental combinations.
The amount of perturbation for each combination of drug and clone is shown, with deeper shades of blue indicate larger degrees of perturbation (larger distances from the mean feature values for a clone and drug combination to the mean feature values of the vehicle-only control for that clone; feature values are contained in Supplementary file 4). Images were subjected to additional quality control for this analysis; diagonal red lines mark experiments failing this stricter quality control. Clones are grouped by the labels assigned to the unperturbed (control) subcellular localization patterns; the mean perturbation of all proteins with a given label is also displayed for each drug. Drugs were clustered with average linkage using the mean perturbation data. Different tagged variants of the same protein (labeled with clone identifier in parenthesis) sometimes have distinguishably different responses to drugs (e.g. Rps27a clones 29 and 44). Beyond cytotoxic conditions (e.g. Latrunculin B at 1.25 mM) few dominating patterns are apparent; neither unperturbed subcellular compartment nor known targets of drugs are major predictors of the degree of perturbation of most experiments.