(A) Representative examples of homotopic CLE grafts (numbers identify individual embryos in B–D). Cell fate in the rostral CLE in rostral-to-caudal (Aa–l) and medial-to-lateral direction (Am–t). Cell fate in the caudal-most lateral epiblast (Au–x). Arrows show the notochord; white boxes the CNH. lvm, lateral and ventral mesoderm; nt, neural tube; pxm, paraxial mesoderm; tbm, tail bud mesoderm. (B–D) Diagrams indicate the graft type performed, the shading within them, the predominant prospective fate of each region (pink, mesoderm; light blue, neuromesodermal; dark blue, neural; light brown, lateral/ventral mesoderm). Graphs display single grafted embryos and their contribution in the differentiated axis to the neurectoderm (N) and mesoderm (PXM) or both. Numbers below the bars indicate the graft series followed by an individual embryo identifier (e.g. embryo 1.01). Below the x-axis, graft cell contribution in the TB (dorsal part of the CNH, ventral (notochordal) part of CNH and TBM), is represented by a black square, grey square or grey circle, respectively. (B) Fate in L1, L2 and L3. L1 grafts gave rise to two distinct contribution patterns, L1A (axis only) and L1AT (axis and tail bud). (C) Fate in either the medial or lateral half of L1 or L2. One graft (embryo 2.06) contributed to the neural crest (NC). (D) Grafts of L/St5 contributed entirely to the lateral/ventral mesoderm (LVM). Unilateral versus bilateral PXM contribution is shown in Figure 6—figure supplement 4.
DOI:
http://dx.doi.org/10.7554/eLife.10042.010
Figure 6—source data 1. Presomitic mesoderm contamination in CLE grafts.Additional information and analysis to show the majority of paraxial mesoderm in CLE grafts is derived from NMPs rather than contaminating PSM progenitors that were co-grafted.