Fig. 1.

PARP-1 detection in human lens cells. (A) Fluorescent micrographs showing PARP-1 distribution in FHL124 cells and the native human lens epithelium. (B) PARP-1 expression in the lens epithelium and pre-denucleating lens fibre cells. A strong nuclear expression of PARP-1 is observed in cells exhibiting typical nuclei. However, as fibre cells become more embedded in the lens, chromatin appears condensed and these cells do not exhibit PARP-1 (arrowed). The data presented are representative of four lenses from individual donors that were sectioned and stained. As a point of reference a cross-sectional diagram of the lens has been included (adapted from Maidment et al. [44]).