Table 2.

Transgenic N. benthamiana lines vary in transgene copy number, gene expression and growth

Line no.	Transgene copy number		Relative transgene expressiona		Morphology (T0 transformants vs. wild type)b	Germination ratec
	AtLecRK-I.9/IX.1	NPTII	AtLecRK-I.9	AtLecRK-IX.1
EV−
1	0	1	n.d.	n.d.	–	100
2	0	2	n.d.	n.d.	–e	100
3	0	5	n.d.	n.d.	–	100
4	0	2	n.d.	n.d.	–e	100
I.9-OE−
1	1	1	5.0		Slightly smallere	32
2	2	2	5.6		Smaller plants with curly leaves	60
3	3	3	6.2		Smaller plants with compacted rosettee	40
4	3	3	1.0		–	60
5	1	1	3.7		–e	100
6	4	4	7.8		–	55
7	0	1	n.d.		–e	/d
8	1	1	11.1		–	60
9	1	1	2.8		Smallere	85
10	5	6	59.4		Smallere	95
11	4	6	21.7		Smallere	65
12	1	1	4.1		Smaller plants with compacted rosette, thick leavese	55
13	2	2	3.9		–	50
14	2	2	3.6		–	70
15	1	1	2.3		Smallere	60
16	1	1	81.9		Smaller plants with compacted rosette	35
17	3	3	47.3		Slightly smaller and curly leaves	85
IX.1-OE−
1	2	2		1.7	–e	100
2	1	2		3.7	Smaller plants with curly round leavese	100
3	1	2		9.0	Smallere	100
4	2	2		2.0	–e	100
5	2	3		2.0	–e	100
6	1	2		2.4	–	100
7	1	1		11.2	Smallere	100
8	2	2		1.0	–	100
9	1	1		3.4	–	100
10	1	1		63.2	Smaller plants with old leaves showing necrosise	100
11	1	1		87.3	Smaller plants with old leaves showing necrosis	100
12	1	1		90.3	Smaller plants with old leaves showing necrosise	100

^a n.d. not detectable

^b –, no difference compared with wild-type N. benthamiana

^cPercentage of germinated seeds of T₁ progeny lines (n = 18–24) after 3 days on MS

^d / not tested

^eSimilar morphology in T₁ progeny lines harboring the transgene