Fig. 4.

Physical maps of the 12 potato chromosomes, showing to the right of each chromosome the genomic positions of the markers genotyped in the QUEST population, and to the left markers linked to previously mapped QTL for tuber starch content (TSC, specific gravity (SG) in Bonierbale et al. 1993), yield (TY), starch yield (TSY), tuber weight (TW), tuber number (TN), and tuberization (TZ, in vitro tuberization (ivt) and greenhouse tuberization (gt) in Šimko et al. 1999). Numbers in parenthesis after the markers are numerical codes for the corresponding reference: 1 (Bonierbale et al. 1993), 2 (Schäfer-Pregl et al. 1998), 3 (van den Berg et al. 1996), 4 (Šimko et al. 1999), 5 (Zhou et al. 2014), and 6 (Navarro et al. 2011). RFLP marker sequences were retrieved from the databases Sol Genomics Network (TG, CT, and CD markers, http://www.sgn.cornell.edu/) and GABI Primary Database (GP and CP markers, GluA, SK2, SbeI, AGPaseB, pat, prp1, http://www.gabipd.org/). Microsatellite primer sequences were retrieved from the literature cited in Materials and Methods. Gene sequences were obtained from GenBank entries (http://www.ncbi.nlm.nih.gov/). Sequences were BLASTed against the potato genome sequence (pseudomolecules v4.03, http://potato.plantbiology.msu.edu/blast.shtml). QTL are shown red next to the linked or associated marker locus or next to the brackets indicating genome segments harbouring the QTL. Loci associated with TSC, TY, TSY, TW, and/or TN in the QUEST(+LAN) population but not in the QUEST(−LAN) population (MM-PK model, p < 0.01, MAF >1 %, see Online Resource 5) are shown in blue letters. Loci associated with TSC, TY, and/or TSY in both the QUEST(−LAN) and QUEST(+LAN) population are shown in green letters (see Table 5). Genes with known function are in italics