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In vivo regulation of Wnt5a and Wnt11 mRNA levels in GCs by gonadotropins. Immature (21- to 26-d-old) female mice were injected with eCG (5 IU, i.p.) 44-48 h before the administration of hCG. GCs were isolated by needle puncture. mRNA levels were determined by RT-qPCR and normalized to the housekeeping gene Rpl19 (n = 4 animals/time point). Data are means ± sem. Different letters show statistically significant differences between strains. P < 0.05.
