Figure 4.

Progressive follicle loss and increased follicular atresia in the ovaries of Wnt5a^flox/−;Amhr2^cre/+ mice at 5 d (A), 42 d (B), and 8 mo (C). Ovaries (n = 6 per genotype at each age) were serially sectioned, and all follicles from every fifth section were counted; categorized as primordial, primary, secondary, or antral; and scored as healthy or atretic. Data represent raw follicle counts and were not adjusted to estimate the total ovarian follicle population. Data are means ± sem. Different letters above histograms indicate statistically significant differences between strains. P < 0.05.