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RT-qPCR confirmation of microarray data. Primary cultured GCs were treated or not with 3.5 μg/ml WNT5a for 3 h (n = 4 samples/group), and mRNA levels of the indicated genes were determined by RT-qPCR and normalized to the housekeeping gene Rpl19. Data are means ± sem. **P < 0.01 and ***P < 0.001, statistically significant differences in the effect of WNT5a treatment.
