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. 2015 Dec 20;30(4):1670–1682. doi: 10.1096/fj.15-279802

Figure 3.

Figure 3.

CD siRNA inhibits increased permeability induced by activated U937 cells. A) Representative Western blot image of CD and β-tubulin protein in PMA-stimulated U937 cells (U937 Mϕ; Mac), U937 Mϕ treated with CD siRNA, and U937 Mϕ treated with scrambled siRNA. B) The CD beads from U937 Mϕ cells treated with siRNA (siRNA CD beads) caused a significant reduction in the degree of induced HREC barrier alteration as compared to the CD beads from untreated U937 Mϕ cells (CD beads). *P = 0.005, significantly greater than untreated U937 Mϕ cells (CD beads). C) Representative image of VE-cadherin (cad) immunofluorescence in HRECs grown in the presence of CD beads or siRNA CD beads. siRNA CD beads induced fewer gaps between adjacent cells. White arrows indicate gaps between adjacent cells treated with CD beads. *P = 0.0001, significantly greater than cells grown in Ctrl; **P = 0.0005, significantly less than HRECs grown in the presence of CD beads.