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. 2015 Dec 28;30(4):1590–1598. doi: 10.1096/fj.15-283226

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Western blot analysis of mAb Guy’s 13 expressed in N. tabacum. Samples from mAb expressed transgenically or transiently in N. tabacum were separated on nonreducing 4–15% SDS-PAGE gels, and proteins were blotted onto nitrocellulose membrane. For detection, anti-Fc (A, lanes 1–3) and anti-κ (B, lanes 4–6) antisera were used. Lanes 1 and 4, nontransgenic N. tabacum (WT); lanes 2 and 5, crude leaf extract of transgenic N. tabacum plants expressing Guy’s 13 (transgenic); lanes 3 and 6, crude leaf extract from transiently expressed mAb Guy’s 13 (transient). Asterisk represents fully assembled antibody. Letters a–f represent antibody fragments.