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N-terminal sequencing results of nonreduced mAb Guy’s 13 fragments. N-terminal sequencing of protein A/G–purified mAb Guy’s 13 blotted onto PVDF membrane and stained with Coomassie G250. Arrows indicate position of N-terminal sequence within heavy and light chain amino acid sequence. Uppercase letters indicate sequence returned from N-terminal sequencing by Edman degradation; lowercase letters indicate major antibody fragments.
