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. 2015 Dec 28;30(4):1590–1598. doi: 10.1096/fj.15-283226

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Transient expression of Guy’s 13 heavy and light chain mutants in N. tabacum. Nonreducing Western blot analysis of leaf extracts from transiently expressed Guy’s 13 heavy chain mutants (HC1, HC2, and HC4) expressed together with nonmutated Guy’s 13 light chain (LC) and light chain mutants (LC1, LC3, LC5, and LC8) together with nonmutated Guy’s 13 heavy chain (HC). Detection was with HRP-conjugated antimurine κ chain antiserum. Control was nonmutated Guy’s 13 heavy and light chains (HC + LC). Asterisk indicates fully assembled IgG. Letters a–f indicate major antibody fragments.