TABLE 1.

Primer sequences for gene expression studies

Primer	Sequence (F)	Sequence (R)	Product size (bp)
Tfam	AGCTGAGTGGAAAGCATACAAA	CCTTCTCCATACCCATCAGC	83
Nrf2	AGCAAGACTTGGGCCACTTA	CTGAGCCGCCTTTTCAGTAG	97
Pgc-1α	TTACACCTGTGACGCTTTCG	TTGCTTCCGTCCACAAAAGT	97
MnSOD	GTGTGGGAGCACGCTTACTA	TCTCCCAGTTGATTACATTCCA	85
CuZnSOD	AGATGACTTGGGCAAAGGTG	AATCCCAATCACTCCACAGG	85
Ccs	CCTTTTCCAGAACCCCAAG	CTCGCTCCTCCCAGATAGTG	66
ECSOD	ATCCACGTGCATGAGTTCG	ACCTCCATCGGGTTGTAGTG	74
Catalase	CCCCCAACTATTACCCCAAC	TGAAGCGTTTCACATCTACAGC	96
Gpx1	ACCCGCTCTTTACCTTCCTG	ACACCGGAGACCAAATGATG	99
Gpx4	CCGGCTACAACGTCAAGTTT	CCTTGGGCTGGACTTTCAT	96
Gr	GGGATTGGCTGTGATGAGAT	GAATGGCAACTGTGTTGTCG	91
Hmox1	AGACACCCCGAGGGAAAC	GAGAGTGAGGACCCACTGGA	74
Prdx1	CACGGAGATCATTGCTTTCA	CCAATCACTTGGCAGTTGAG	66
Prdx3	TCGTCAAGCACCTGAGTGTC	CTCCATGGGTCTCCACAAAC	99
Txnrd1	CAAACGCTGGAGAGGTGAC	TGTCCAGCTGCTGCTTAGTC	72
Nox4	TTGATGGTCCATTTGGAAGC	CCTCCAGCCACACAGAGACT	70
Xo	TAACGCCAAACAGCTCTTCC	CAAGCGTTTCGGATCTTCTC	63
Nf-kb	GCTACACAGAGGCCATTGAAG	AGAGGCAGACAGTGGACCTG	100
Alox12	CCAGCTCCAACACTGTTCCT	AGCTCCTGCAGTTGGAAATC	95
Alox15	CCAGCATTCAGAGGAACACTT	CAGCCAGCTCCTCTCTGAAC	74
Ppar-γ	ACAGACCTCAGGCAGATCGT	GGGTGAAGGCTCATGTCTGT	83
Ltb4r1	GGCTTCGTGGTCAAGCTACT	GACAGGCAGGTGTGTCCTTC	100
Ptgs2	GGAGCACCATTCTCCTTGAA	TAAAACCCACTTCGCCTCCA	94
p53	TTTGAGGTTCGTGTTTGTGC	CCTTTTTGCGGAAATTTTCTT	70
p21	AGGCCCAGTACTTCCTCTGC	GCTCAGACACCAGAGTGCAA	88
p16INK	GACACGCTGGTGGTGCTG	TTGATGTCCCCGCTCTTG	99
Ogg1	GCTTAATGGCCCTTGACAAA	TTAGGATGCCAGCCGTAGTC	88
Casp3	ACGCGCACAAGCTAGAATTT	GAAGGACTCGAATTCCGTTG	74
Col1A1	CACTGCAAGAACAGCGTAGC	CCTCTGAGCTCGATCTCGTT	95
IL6	TCCTTCCTACCCCAATTTCC	GCCACTCCTTCTGTGACTCC	63
IL1-β	CAAGAGCAAAGTGGAGTTTGAG	CTTGTGCTCTGCTTGTGAGG	73
Ccl2	TGCCCTAAGGTCTTCAGCAC	TGTGGAAAAGGTAGTGGATGC	79
Tnfα	CGACTACGTGCTCCTCACC	ACGGCAGAGAGGAGGTTGAC	78
Tgfβ1	TGCCCTCTACAACCAACACA	CTTGCGACCCACGTAGTAGA	100
Fth1	TGAGTGAACAGGTGAAATCCA	TCTTGCGTAAGTTGGTCACG	60
Myh6	AGGCCAACACCAACCTGTC	CAGCTTGTTGACCTGGGACT	98
Ppia	CGGAGAGAAATTTGAGGATGA	TGTGTTTGGTCCAGCATTTG	85

Ccl2, chemokine (C-C motif) ligand 2; ECSOD, extracellular superoxide dismutase; F, forward; Fth1, ferritin heavy chain; Gr, glutathione reductase; Gpx1, glutathione peroxidase 1; Gpx4, glutathione peroxidase 4; Hmox1, hemoxygenase 1; IL6, interleukin 6; IL1-β, interleukin-1 β; Ltb4r1, leukotriene β 4 receptor 1; Nox4, NADPH oxidase 3; Ogg1, oxoguanine glycosylase 1; Ppia, peptidylprolyl isomerase A (cyclophilin A); Prdx1, peroxiredoxin 1; Prdx3, peroxiredoxin 3; Ptgs2, prostaglandin endoperoxide synthase 2; p16^INK, cyclin dependent kinase inhibitor 2A; R, reverse; Tgfβ1, transforming growth factor β; TNF-α, tumor necrosis factor α; Txnrd1, thioredoxin; reductase 1; Xo, xanthine oxidase.