Table 1.
Primers used for real-time RT-PCR
| Subtype | Product size (bp) | Primer sequence (forward/reverse) | Annealing temperature (°C) |
|---|---|---|---|
| Kv4.2 | 154 | 5’-ATgCAgAgCAAACggAATggT-3’ | 60 |
| 5’CgTCCACAAACTCgTgATTCg-3’ | |||
| Kv4.3 | 230 | 5’-AgCTgATTgTCCTCAACgTgA-3’ | 60 |
| 5’-gTCgTCgTAggCAgAgATgC-3’ | |||
| BKCa-α | 191 | 5’-AgCAATATCCACgCgAACCAT-3’ | 60 |
| 5’-AAAgCCCACCACATgCgTT-3’ | |||
| BKCa-β1 | 80 | 5’-AAggTCAgAgCCAAATTCCAAg-3’ | 60 |
| 5’-AATAggACgCTggTTTCgTTC-3’ | |||
| GAPDH | 119 | 5’-CTCTgCTCCTCCTgTTCgAC-3’ | 60 |
| 5’-ACgACCAAATCCgTTgACTC-3’ |
RT-PCR: Reverse-transcription polymerase chain reaction; BKCa: large conductance calcium- and voltage-sensitive K+ channel; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Kv: Voltage-gated potassium channel.