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. 2016 Feb 5;129(3):295–303. doi: 10.4103/0366-6999.174489

Figure 3.

Figure 3

IL-4/13 and IFN-γ regulate the expression of proteases involved in filaggrin processing in cultured keratinocytes. KLK7 (a), KLK5 (b), matriptase (c), CAP1 (d), and caspase 14 (e) messenger RNA were measured by real-time (quantitative) PCR, *P < 0.05. (f) Protein expression was evaluated by Western blotting. HaCaT in LC (1.3 mmol/L CaCl2) or HC (10.0 mmol/L CaCl2) culture medium; NHK in LC (0.09 mmol/LCaCl2) or HC (1.3 mmol/L CaCl2) culture medium (n =/three experiments in duplicate). IL-4/13: Interleukin-4/13; IFN-γ: Interferon-γ; LC: Low calcium; HC: High calcium; NHK: Normal human keratinocyte; KLK7: Kllikrein 7; KLK5: Kllikrein 5; CAP1: Channel-activating serine protease 1; PCR: Polymerase chain reaction.