Table 1.

The primer sequences of target and housekeeping genes for quantitative RT-PCR

Genes	Sequences of primers	Size of amplification fragments (bp)
β-actin	FW: 5’-TGGCACCCAGCACAATGAA-3’	186
	Rev: 5’-CTAAGTCATAGTCCGCCTAGAAGCA-3’
FLG	FW: 5’-AGTCACGTGGCAGTCCTCACA-3’	143
	Rev: 5’-TCTAAACCCGGATTCACCATAATCA-3’
Matriptase	FW: 5’-TCTGAGCAGTGGGTGCTGTC-3’	98
	Rev: 5’-CTCGGAGTAGGAGTCTAGCTGGTG-3’
CAP1	FW: 5’-AAGGGCAACCCTGAGTGTGA-3’	105
	Rev: 5’-CGAGCCTGTCTCGTGAATGA-3’
SPINK5	FW: 5’-GAATTTCGGGACCAAGTGAGGA-3’	113
	Rev: 5’-ACACTGGCACACATGGCACA-3’
KLK5	FW: 5’-TGCTAAGGCCCAACCAGCTC-3’	149
	Rev: 5’-GAACATCTGCTGCCCAGATTCA-3’
KLK7	FW: 5’-CTCTGACCTCATGTGCGTGGA-3’	166
	Rev: 5’-CAGGGTACCTCTGCACACCAAC-3’
Caspase14	FW: 5’-GACCTGGATGCTCTGGAACACA-3’	119
	Rev: 5’-GAATCGATGGCCTGCTGGA-3’

FW: Forward primer; Rev: Reverse primer; FLG: Filaggrin coding gene; CAP1: Channel-activating serine protease 1; SPINK5: Serine protease inhibitor Kazal-type 5 gene; KLK: Kallikrein; RT-PCR: Real-time polymerase chain reaction.