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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cancer Res. 2016 Jan 12;76(5):1135–1145. doi: 10.1158/0008-5472.CAN-15-2272

Figure 3.

Figure 3

NOD1 suppresses Cdx2 expression induced by H. pylori (Hp) infection. A, total RNA extracted from AGS cells transfected with the indicated siRNA and infected with H. pylori was subjected to RT-PCR. B, AGS cells were cotransfected with the indicated siRNA, cdx2-luc reporter vector, and pRL-TK plasmid; cell lysates were subjected to luciferase assay. C, total RNA extracted from GSM06 cell stably expressing either control shRNA or NOD1-shRNA was subjected to qPCR. D, GSM06 cells stably expressing NOD1-shRNA were infected with 5 × 107 CFU/mL H. pylori. Total RNA was extracted and subjected to qPCR. E, GCIY cells were infected with H. pylori with or without preincubation with iE-DAP. Total RNA was extracted and subjected to qPCR. Results shown in B–E indicated as means ± SD. *, P < 0.05 as compared with H. pylori-infected cells transfected with control siRNA (B), control shRNA transfected cells (C and D), and non-pretreated cells (E). AU, arbitrary units.