Skip to main content
. 2016 Jan 6;213(8):1271–1279. doi: 10.1093/infdis/jiv557

Figure 4.

Figure 4.

Addition of Shiga toxin 1a (Stx1a) 3 hours prior to Stx2a intoxication amplified the cointoxication morbidity and survival benefits. A, Mean percentage weight change over time. B, Mouse survival over the course of the experiment. Survival was equivalent after Stx2a intoxication, whether mice received phosphate-buffered saline (PBS) 3 hours prior to or at the time of intoxication (P = .07). Delivery of Stx2a 3 hours prior to Stx1a also resulted in survival equivalent to that of Stx2a delivery alone (P = .19). Stx1a intoxication 3 hours prior to Stx2a intoxication significantly improved survival as compared to concurrent cointoxication (P = .016). When delivered 3 hours prior to Stx2a, Stx1a and SW09 equivalently extended survival (P = .16). Stx1a preincubation with either cαStx1 or human immunoglobulin G (huIgG) had no effect on survival as compared to Stx1a, delivered 3 hours prior to Stx2a (P = .69 and P = .76, respectively). The results represent 1–2 biological replicates with 8–16 mice per group. The plus sign indicates cointoxication at same time; the arrow indicates 3 hours between Stx intoxication.