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. 2015 Nov 24;213(8):1280–1288. doi: 10.1093/infdis/jiv562

Figure 1.

Figure 1.

Dendritic cell (DC)–specific Toll-like receptor 4 (TLR4) regulates bacterial clearance and thus regulates systemic inflammation and survival after cecal ligation and puncture (CLP). Wild-type (WT), global Tlr4-/-, Flox (cell-specific Tlr4-/- control), and DC-Tlr4-/- mice were subjected to CLP without antibiotic treatment. A, Seven-day survival after CLP. Data are from 20 mice per group from 2 separate experiments. Statistical difference was tested using the log-rank test. B and C, Blood and peritoneal lavage fluid (PLF) were collected at 18 hours after CLP. Bacterial counts in blood (B) and peritoneal lavage fluid (C). Data are from 8 mice per group from 2 separate experiments. Statistical difference was tested using a nonparametric Mann–Whitney U statistics. D, Plasma cytokine level. Blood was collected 4 and 18 hours after CLP. Plasma tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), interleukin 12p70 (IL-12p70), and interleukin 10 (IL-10) concentrations were measured by enzyme-linked immunosorbent assay. Data are means ± SD from 8 mice per group from 4 separate experiments. Statistical difference was tested using a 2-tailed paired t test. Abreviation: CFU, colony-forming units.