Figure 3.

Adoptive transfer of wild-type dendritic cells (DCs) impairs bacterial clearance and polymorphonuclear leukocyte (PMN) recruitment in DC-Tlr4^-/- mice after cecal ligation and puncture (CLP). DC-Tlr4^-/- mice were subjected to CLP without antibiotic administration. Immediately after CLP, DC-Tlr4^-/- mice were intraperitoneally injected with wild-type (WT) DCs or Tlr4^-/- DCs (0.5 million cells/mouse). Mice injected with WT DCs, Tlr4^-/- DCs, or phosphate-buffered saline (PBS) were subjected to CLP. Peritoneal lavage fluid (PLF) and plasma were collected 18 hours after CLP. Control mice (Cont) are mice only subjected to injection. A, Bacterial counts in PLF. Data are from 6 mice per group from 2 separate experiments. Statistical difference was tested using a nonparametric Mann–Whitney U test; *P < .05 vs Tlr4^-/- DCs and PBS. B and C, PMNs in PLF were analyzed by flow cytometry for total number of PMNs (B) and mean fluorescence intensity (MFI) for CXCR2 expression (C). D, Circulating cytokine and chemokine levels. Plasma interleukin 6 (IL-6), CXCL1, interleukin 10 (IL-10), and interleukin 12p70 (IL-12p70) concentrations were measured by enzyme-linked immunosorbent assay. Data are means ± SD from 3 mice per control group and 6 mice per CLP group. Statistical difference was tested using the 2-tailed unpaired t test. Abreviation: CFU, colony-forming units.