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. 2015 Nov 24;213(8):1280–1288. doi: 10.1093/infdis/jiv562

Figure 4.

Figure 4.

Cecal ligation and puncture (CLP) with antibiotics reveals a role for dendritic cell (DC)–specific Toll-like receptor 4 (TLR4) in interleukin 10 (IL-10) release in polymicrobial sepsis. Mice were subjected to CLP with the administration of the antibiotic Primaxin (25 mg/kg, subcutaneously starting 2 hours after CLP and then every 12 hours). Peritoneal lavage fluid (PLF) and plasma was collected 18 hours after CLP. Control mice (Cont) are out-of-box mice. A and B, Polymorphonuclear leukocyte (PMN) count (A) and mean fluorescence intensity for CXCR2 expression on peritoneal PMNs (B) were measured by flow cytometry. C, Circulating cytokine and chemokine level. Plasma interleukin 6 (IL-6), CXCL1, CXCL2, CXCL2, interleukin 10 (IL-10), and interleukin 12p70 (IL-12p70) concentrations were measured by enzyme-linked immunosorbent assay. Data are means ± SD for 3 mice per control group and 6 mice per CLP group. Statistical difference was tested using a 2-tailed unpaired t test.