Skip to main content
. 2015 Nov 24;213(8):1280–1288. doi: 10.1093/infdis/jiv562

Figure 5.

Figure 5.

Dendritic cell (DC)–specific Toll-like receptor 4 (TLR4) regulates CXCR2 expression in polymorphonuclear leukocytes (PMNs) via interleukin 10 (IL-10) after lipopolysaccharide (LPS) stimulation in vitro. DCs (CD11c+) and PMNs (Ly6G+) were isolated from spleen and stimulated with LPS (100 ng/mL) or polyinosinic-polycytidylic acid (PIC; 10 µg/mL) for 4 hours. A, The relative expression of Cxcr2 was quantitated by real-time reverse-transcription polymerase chain reaction analysis. B, IL-10 concentration in medium was measured by enzyme-linked immunosorbent assay. C, PMNs cocultured with wild-type (WT) DCs or Tlr4-/- DCs in the presence of LPS (100 ng/mL) were treated with IL-10 antibody (1 mg/mL) or recombinant IL-10 (20 ng/mL), respectively. PMNs cocultured with WT DCs treated with immunoglobulin G1 (IgG1) served as a control for IL-10 antibody treatment. Cxcr2 expression was measured by flow cytometry. Data are mean values ± SD from 3 experiments. Statistical difference was tested using by the 2-tailed paired t test. Abbreviation: MFI, mean fluorescence intensity.