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. 2015 Dec 9;213(8):1253–1261. doi: 10.1093/infdis/jiv586

Figure 1.

Figure 1.

Antiviral activity of T-705 against rabies virus (RABV) in mouse neuroblastoma cell lines. A, Expression of HPRT in NA and N2a cells. HPRT and β-actin proteins were detected by Western blots. The relative molecular mass is indicated on the left. B, The 1088 or challenge virus standard (CVS) RABV strain was inoculated into NA or N2a cells at a multiplicity of infection (MOI) of 0.01. The cells were incubated for 96 hours in the presence (1000 µM) or absence (0 µM) of T-705. Each virus titer in the supernatant was determined using the focus assay. C, Expression of HPRT in NA cells transfected with the empty or hprt vector. The indicated proteins were detected by Western blots. D, Efficacy of T-705 against NA cells transfected with the hprt vector. The 1088 strain was inoculated into NA cells transfected with the empty or hprt vector or into N2a cells. The cells were incubated for 96 hours in the presence (1000 µM) or absence (0 µM) of T-705. Each virus titer in the supernatant was determined using the focus assay. Data represent the mean ± SD (n = 3). *P < .05, by the Student t test; **P < .05, by 2-way analysis of variance for interaction. Abbreviations: FFU, focus-forming units; NS, not significant.