Fig. 3.
Human brain tumor explants express a HIF-2αHi phenotype which diminishes with time. Human brain tumor explants were recovered from surgery and depleted of CD11b+ cells. Tumor cells were plated at 1 × 104 per well either alone (24 h CD11b−) or with 5 × 103 CD11b+ cells (24 h CD11b+). HIF-2α expression was measured at 24 h. In cultures from which tumor cells survived more than a week, HIF-2α was measured from 1 × 104 tumor cells after 2 weeks, by which time CD11b+ cells had been washed away/died (2 wk CD11b-). HIF-2α was also measured from 1 × 103 separated CD11b+ cells 24 h after explant. Results are representative of 3 separate measurements. Error bars are expressed as SD.