Table 2.
Gene | Primer | Annealing temperature (°C) | Product size (bp) |
---|---|---|---|
Sheep COL1A1 | Forward: AGG GAC CCA AAG GAG ACA CT | 55 | 198 |
Reverse: GCA CGG AAA TTC CTG TTG AT | |||
Sheep COL2A1 | Forward: CGT CAC CTA CCA CTG CAA GA | 58 | 219 |
Reverse: GGG AGA CGT GAG GTC TTC TG | |||
Sheep COL3A1 | Forward: CTT TTC GCT CTG CTT CAT CC | 55 | 216 |
Reverse: TTC TCC AAA CGG GAT TTC AG | |||
Sheep VCAN | Forward: CCTGTTGTAGAAAATGCCAAGACC | 58 | 344 |
Reverse: GTTAGGACTTTGGCTGAAATGATGA | |||
GAPDH | Forward: CTC ACT GGC ATG GCC TTC CG | 55 | 293 |
Reverse: ACC ACC CTG TTG CTG TAG CC |
COL1A1, collagen type I; COL2A1, collagen type II; COL3A1, collagen type III; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse transcription polymerase chain reaction; VCAN, versican.