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. 2016 Apr 1;13(2):144–151. doi: 10.1089/zeb.2015.1176

Table 2.

Flow Cytometry Analysis of the Percentage of Recognizable Cells and Percentage of Membrane-Intact Cells in Four Extender Solutions at Different Osmolalities During Refrigerated Storage for Fresh Sperm in Zebrafish D. rerio

    30 min 2 h 24 h 48 h 72 h
Extender solution Osmolality (mOsmol/kg) Recognizable cells Membrane integrity Recognizable cells Membrane integrity Recognizable cells Membrane integrity Recognizable cells Membrane integrity Recognizable cells Membrane integrity
HBSS 150 63 ± 10 68 ± 2 68 ± 25 82 ± 4 24 ± 11 71 ± 9 13 ± 1 43 ± 19 5 ± 2 18 ± 11
  300 100 ± 0 91 ± 2 90 ± 9 90 ± 1 82 ± 5 85 ± 3 76 ± 6 75 ± 4 53 ± 15 66 ± 5
  600 90 ± 7 93 ± 1 90 ± 10 91 ± 2 59 ± 29 88 ± 2 35 ± 16 82 ± 7 21 ± 14 75 ± 7
  1200 36 ± 7 97 ± 1 31 ± 8 98 ± 0 12 ± 15 98 ± 0 7 ± 4 97 ± 1 2 ± 3 96 ± 5
Ca2+-free HBSS 150 37 ± 13 73 ± 6 32 ± 18 93 ± 3 21 ± 14 87 ± 11 2 ± 2 48 ± 19 0 ± 0 27 ± 23
  300 100 ± 0 91 ± 2 87 ± 5 92 ± 1 65 ± 19 87 ± 2 37 ± 18 51 ± 14 32 ± 31 18 ± 7
  600 90 ± 8 93 ± 0 81 ± 20 92 ± 1 37 ± 25 54 ± 31 22 ± 16 25 ± 32 16 ± 12 17 ± 27
  1200 38 ± 12 97 ± 1 11 ± 4 98 ± 0 1 ± 1 69 ± 29 0 ± 0 47 ± 17 0 ± 0 17 ± 21
Glucose 150 57 ± 36 69 ± 5 49 ± 49 87 ± 1 16 ± 14 35 ± 18 1 ± 1 10 ± 12 1 ± 0 9 ± 6
  300 100 ± 0 93 ± 2 93 ± 10 90 ± 5 38 ± 18 28 ± 35 13 ± 4 9 ± 14 9 ± 12 2 ± 2
  600 73 ± 7 75 ± 17 68 ± 17 57 ± 25 11 ± 9 6 ± 8 13 ± 11 4 ± 3 4 ± 4 4 ± 3
  1200 53 ± 13 49 ± 21 58 ± 31 32 ± 10 10 ± 5 3 ± 3 2 ± 2 7 ± 2 2 ± 1 12 ± 1
NaCl 150 53 ± 10 58 ± 11 26 ± 2 86 ± 3 12 ± 8 67 ± 25 3 ± 3 40 ± 31 1 ± 1 47 ± 48
  300 100 ± 0 88 ± 2 97 ± 5 89 ± 2 70 ± 6 77 ± 6 63 ± 17 70 ± 8 55 ± 10 67 ± 8
  600 87 ± 17 92 ± 0 93 ± 7 92 ± 0 42 ± 19 87 ± 2 36 ± 17 82 ± 5 28 ± 15 78 ± 4
  1200 36 ± 10 97 ± 1 20 ± 17 98 ± 1 9 ± 5 99 ± 0 6 ± 4 98 ± 1 4 ± 4 77 ± 34