Table 3.
Sperm Cryopreservation for Zebrafish D. rerio from a Farm-Raised Population (n = 20) and the AB Reference Line (n = 20)
| Biological factors | Motility (%) | Post-thaw fertility (%) | Recognizable cells (%) | Membrane integrity (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source | Value | Length (mm) | Weight (g) | Testis (mg) | Fresh | Post thaw | At 4 h | At 24 h | Post equilibration | Post thaw | Fresh | Post equilibration | Post thaw |
| Farm-raised N = 20 | Minimum | 26.66 | 0.363 | 2.7 | 60 | 7 | 4 | 4 | 87 | 30 | 84 | 77 | 35 |
| Maximum | 32.58 | 0.571 | 9.7 | 80 | 50 | 58 | 65 | 100 | 100 | 95 | 91 | 66 | |
| Mean | 30.06 | 0.511 | 7.2 | 68 | 25 | 27 | 32 | 95 | 82 | 92 | 86 | 48 | |
| SD | 1.40 | 0.057 | 1.8 | 8 | 16 | 15 | 18 | 5 | 16 | 3 | 4 | 8 | |
| Research AB Line N = 20 | Minimum | 22.18 | 0.212 | 2.2 | 70 | 10 | 36 | 36 | 83 | 45 | 80 | 88 | 38 |
| Maximum | 28.19 | 0.418 | 6.2 | 85 | 55 | 83 | 100 | 95 | 88 | 94 | 94 | 53 | |
| Mean | 25.02 | 0.312 | 3.6 | 80 | 28 | 62 | 73 | 89 | 74 | 91 | 92 | 45 | |
| SD | 1.25 | 0.053 | 1.0 | 6 | 15 | 14 | 21 | 3 | 9 | 4 | 1 | 4 | |
| p | 0.000 | 0.000 | 0.000 | 0.000 | 0.489 | 0.000 | 0.000 | 0.000 | 0.017 | 0.188 | 0.000 | 0.060 | |
Body length, body weight, testis weight, fresh motility, post-thaw motility, percentage of recognizable cells, and membrane integrity by flow cytometry analysis in fresh, post-equilibration and post-thaw sperm, and post-thaw fertility checked at 4 h and survival at 24 h after in vitro fertilization.
SD, standard deviation.