Figure 7.

Areas of mineralized matrix in different implants as indicated by von Kossa staining (black regions, asterisks). Obviously, the cells/scaffolds treated with the syringe-aided inoculation method (A, D, G) induced more mineralized matrix than did those of the other groups at all time points (B, C, E, F, H, I, J) (mean±SD, *p<0.05). At 2 weeks of implantation, more mineralized nodes were formed in the constructs of Group T when compared with those of Group C, although it was not statistically significant (B, C, J) (p>0.05). After cultured for 4 to 6 weeks, the constructs treated with the two-sided inoculation method showed more mineralization than did those in Group C, with statistics significance (E, F, H , I, J) (mean − SD, *p<0.05). Scale bar 100 μm. Mean optical density of the mineralization nodules of the cells/scaffold compounds was shown in (J). To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/wound