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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Immunol. 2016 Feb 22;196(7):3054–3063. doi: 10.4049/jimmunol.1501797

FIGURE 3. Short-boosted Ag-specific CD8 T cells express granzyme B and protect against viral infection.

FIGURE 3

(A) Splenocytes were isolated at day 5 post 3°. Granzyme B expression is shown on CD44+ Kb-SIINFEKL-specific CD8 T cells from short-boosted mice (black, solid line) and long-boosted mice (grey, dashed line); grey filled histogram indicates gating on CD44 low CD8+ T cells. (B) Spleen and (C) ovaries from 3° short-boosted, 3° long-boosted or 1° VV-OVA challenged mice were isolated at day 5 after VV-OVA to determine vaccinia virus titers. Data are representative of 2 experiments, N=3-4 mice per experiment. Graphs show mean +/− SEM. * = p<0.05.