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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Immunol. 2016 Feb 22;196(7):3054–3063. doi: 10.4049/jimmunol.1501797

FIGURE 5. Short-boosted Ag-specific CD8 T cells have a memory phenotype.

FIGURE 5

(A) Visualization and (B-C) quantification of KLRG1 and CD127 expression on CD44+ Kb-SIINFEKL tetramer+ CD8 T cells from short-boosted (black) or long-boosted (white) mice at day 30 post 3° immunization. Plots are gated on CD44+ Kb-SIINFEKL tetramer+ CD8 T cells from splenocytes. (D) Expression of indicated proteins on CD44+ Kb-SIINFEKL tetramer+ CD8 T cells from short-boosted (black, solid line) and long-boosted (dotted, grey line) mice; grey filled histograms indicate expression on CD44 low CD8+ T cells. Data are representative of at least 2 experiments, N=2-5 mice per experiment. Graphs show mean +/− SEM. ***p=0.0001, and **** p <0.0001.

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