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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Nov 10;1860(5):879–891. doi: 10.1016/j.bbagen.2015.11.005

Figure 4.

Figure 4

Representative ITC binding isotherms and best fits for Fe2+ (left panel) and Zn2+ (right panel) titrations into human serum transferrin (hTf). (A) in the absence of 20 mM bicarbonate and (B) in the presence of 20 mM bicarbonate. The experiments were carried out under anaerobic conditions in the presence of 10 mM dithionite in 50 mM Mops, 50 mM NaCl, pH 7.01 and 25.00 °C. (Reprinted with permission from Ref. 46).