Table 5.

Apparent ITC thermodynamic parameters for Cu⁺¹ binding to Atox1 and five variants of the Wilson protein, WD12, WD34, WD56, WD16, and WD14 in 100 mM MES, 100 mM NaCl pH 6.5 and 22 °C. The ΔH values include contributions from Cu(I) binding and other associated events such as deprotonation of the two cysteines in the CXXC motif and changes in the buffer ionization state. Except for WD14 where an independent two-site model was used to fit the data, all other ITC data were best fitted with a single-site model. WD12, WD34, WD 56 and WD 16 represent WND metal-binding domains 1–2, 3–4, 5–6, and 1–6, respectively. The data are taken from ref. 37.

100 mM MES, 100 mM NaCl pH 6.5 and 22 °C	n	KITC (M−1)	Δ HITC (kcal/mol)	− TΔS (kcal/mol)	ΔG (kcal/mol)
Cu1+ binding to Atox1	1.41 ± 0.22	(2.45 ± 1.23) × 105	− 12.96 ± 1.20	+ 5.6	− 7.36
Cu1+ binding to WD12	0.95 ± 0.38	(2.07 ± 1.57) × 105	− 11.78 ± 4.17	+ 4.52	− 7.26
Cu1+ binding to WD34	2.23 ± 0.19	(4.67 ± 2.72) × 106	− 9.76 ± 3.66	+ 0.65	− 9.11
Cu1+ binding to WD56	1.92 ± 0.02	(1.13 ± 0.21) × 106	− 11.63 ± 0.04	+ 3.37	− 8.26
Cu1+ binding to WD16	4.92 ± 0.66	(1.96 ± 0.56) × 105	− 11.50 ± 0.38	+ 4.27	− 7.23
Cu1+ binding to WD14	n1=3.38 ± 0.04 n1=1.22 ± 0.07	K1= (1.89 ± 2.00) × 106 K1= (4.98 ± 5.46) × 107	ΔH1 = − 2.90 ± 2.25 ΔH2 = − 9.33 ± 1.10	− 5.67 − 1.18	− 8.57 − 10.51