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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Hepatol. 2015 Nov 27;64(4):881–890. doi: 10.1016/j.jhep.2015.11.025

Fig. 3. Marked Ly6G+ neutrophil and Ly6C+ monocyte infiltration and higher CXCL1 and CCL2 levels in the livers of AKT/RAS-transfected mice.

Fig. 3

(A) Dot plots showing the gating of live/CD45+/CD3CD19CD49− cells for detection of neutrophils (CD11b+Ly6G+Ly6Cint) and monocytes (CD11b+Ly6G−Ly6Chi) in MYC and AKT/RAS tumors. (B) 7/4 antigen immunoreactivity (brown) in the livers of MYC and AKT/RAS mice at 3 dpi and in tumors (50 dpi). Scale bar=100 μm. (C) Heat map representing cytokine/chemokine expression in normal livers and MYC and AKT/RAS liver tumors, measured by protein array (n=1 sample per condition). (D) Quantitative ELISA measurements of CXCL1 and CCL2 in the livers of saline-injected, and AKT/RAS- or MYC-transfected mice. Data are displayed as mean ± SEM with n≥4 mice/group. Statistical significance was calculated using unpaired t-test.