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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: J Immunol. 2016 Jan 11;196(4):1822–1831. doi: 10.4049/jimmunol.1502206

Figure 6. CD8+ T cells retain cytolytic activity in the absence of IL-12, type 1 IFN, or IL-27 signaling.

Figure 6

(a) Schema for the production of 4:1 MBMCs in which CD8+ T cells are either WT or KO, but the other hematopoietic cells are ~80–100% WT. (b and c) In vivo cytolytic activity of TB10.44-11-specific CD8+ T cells in the lungs (b) and spleens (c) of infected 4:1 chimeras 6 weeks post-infection. (d) The total number CD8+ TB10.44-11/Kb tetramer+ (Tetramer+) T cells in the spleens of the indicated 4:1 chimeras 6 weeks post-infection. (E) Nonlinear regression analysis of TB10.44-11-specific CD8+ T cell number vs. specific killing comparing WT to IL-12R−/− 4:1 chimeras. Both data sets are best fit by a single curve (R2=0.8601). Specific killing was calculated as described in the Materials and Methods. Each bar represents the mean ± SEM (n = 4-5 mice per group) *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired Student's t-test). Data are representative of two independent experiments.