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. 2016 Feb 17;147(4):488–498. doi: 10.1111/imm.12583

Figure 6.

Figure 6

miR‐140‐5p regulates expression of T‐bet in CD4+ T cells upon human MOG 35–55 peptide stimulation. Peripheral blood mononuclear cells (PBMCs) from patients with remitting multiple sclerosis (MS) were transfected with mimic oligonucleotide of miR‐140‐5p (mimic) or negative control oligonucleotide (mock), and then cultured with hMOG 35–55 peptide (30 μg/ml) for 20 hr. Intracellular production of T‐bet and GATA3 in CD4+ T cells was measured using flow cytometry. The dot plots are representative of three independent experiments. The histogram showed mean ± SEM from the three experiments. The gate was set on CD4+ T cells.