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. 2016 Feb 2;147(4):414–428. doi: 10.1111/imm.12571

Figure 6.

Figure 6

Messenger RNA and protein expression levels of recombinant interleukin‐17A (rIL‐17A) ‐treated RAW264.7 macrophages and supernatant. RAW264.7 macrophages were treated with different concentrations of rIL‐17A for 24 hr. Cell lysate and cytokine concentrations in the culture medium were detected. The mRNA expression of inducible nitric oxide synthase (iNOS), tumour necrosis factor‐α (TNF‐α), IL‐10 and CD206 were detected by real‐time RT‐PCR using the ΔΔCT method and endogenously normalized to cyclophilin A (a–d) (Student–Newman–Keuls method). Protein levels of granulocyte–macrophage colony‐stimulating factor (GM‐CSF) (e), IL‐2 (f), keratinocyte‐derived chemokine (KC) (g), IL‐5 (h), IL‐9 (i), regulated upon activation normal T‐cell expressed and secreted (RANTES) (j), and vascular endothelial growth factor (VEGF) (k) were detected by the Quantibody Cytokine Array Kit. The 0 ng/ml rIL‐17A‐treated groups served as controls (Student's t‐test). NO concentration in supernatant was determined by measuring the accumulation of nitrite using Griess reagent (l) (Student–Newman–Keuls method). The values represented mean ± SD of three independent experiments (n = 5/group, *P < 0·05, **P < 0·01).